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pljm1 lentiviral transfer plasmid  (Addgene inc)
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Addgene inc pljm1 lentiviral transfer plasmid
Pljm1 Lentiviral Transfer Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pljm1 lentiviral transfer plasmid - by Bioz Stars, 2026-03
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lentiviral transfer plasmid pljm1  (Addgene inc)
96
Addgene inc lentiviral transfer plasmid pljm1
Lentiviral Transfer Plasmid Pljm1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviral transfer plasmid pljm1/product/Addgene inc
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lentiviral transfer plasmid pljm1 - by Bioz Stars, 2026-03
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pljm1 transfer plasmid  (Addgene inc)
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Addgene inc pljm1 transfer plasmid
CAR cassettes and restriction cloning of the HuH85-encoding fragment. a : A simplified schematic of the H85CAR construct which is modified for the insertion of the HuH85-encoding DNA fragment only to develop the HuH8CAR construct. b : PCR-amplification of the HuH85-encoding fragment electrophoresed on 1% agarose gel. Lane 1: PCR amplicon (380 bp); Lane M: 100 bp DNA marker. c : Enzymatic digestion of the <t>HuH85CAR-pLJM1</t> vector electrophoresed on 1% agarose gel for the verification of the HuH8CAR cassette construction using KpnI. Lane 1: KpnI-digested HuH85CAR-pLJM1 vector; Lane M: 1000 bp DNA maker; Lane 2: undigested HuH85CAR-pLJM1 vector
Pljm1 Transfer Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pljm1 transfer plasmid/product/Addgene inc
Average 96 stars, based on 1 article reviews
pljm1 transfer plasmid - by Bioz Stars, 2026-03
96/100 stars
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pljm1 bcli lentivirus transfer vector  (Addgene inc)
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Addgene inc pljm1 bcli lentivirus transfer vector
CAR cassettes and restriction cloning of the HuH85-encoding fragment. a : A simplified schematic of the H85CAR construct which is modified for the insertion of the HuH85-encoding DNA fragment only to develop the HuH8CAR construct. b : PCR-amplification of the HuH85-encoding fragment electrophoresed on 1% agarose gel. Lane 1: PCR amplicon (380 bp); Lane M: 100 bp DNA marker. c : Enzymatic digestion of the <t>HuH85CAR-pLJM1</t> vector electrophoresed on 1% agarose gel for the verification of the HuH8CAR cassette construction using KpnI. Lane 1: KpnI-digested HuH85CAR-pLJM1 vector; Lane M: 1000 bp DNA maker; Lane 2: undigested HuH85CAR-pLJM1 vector
Pljm1 Bcli Lentivirus Transfer Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pljm1 bcli lentivirus transfer vector/product/Addgene inc
Average 96 stars, based on 1 article reviews
pljm1 bcli lentivirus transfer vector - by Bioz Stars, 2026-03
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cmvegfp lentiviral transfer plasmid  (Addgene inc)
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Addgene inc cmvegfp lentiviral transfer plasmid
CAR cassettes and restriction cloning of the HuH85-encoding fragment. a : A simplified schematic of the H85CAR construct which is modified for the insertion of the HuH85-encoding DNA fragment only to develop the HuH8CAR construct. b : PCR-amplification of the HuH85-encoding fragment electrophoresed on 1% agarose gel. Lane 1: PCR amplicon (380 bp); Lane M: 100 bp DNA marker. c : Enzymatic digestion of the <t>HuH85CAR-pLJM1</t> vector electrophoresed on 1% agarose gel for the verification of the HuH8CAR cassette construction using KpnI. Lane 1: KpnI-digested HuH85CAR-pLJM1 vector; Lane M: 1000 bp DNA maker; Lane 2: undigested HuH85CAR-pLJM1 vector
Cmvegfp Lentiviral Transfer Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cmvegfp lentiviral transfer plasmid/product/Addgene inc
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cmvegfp lentiviral transfer plasmid - by Bioz Stars, 2026-03
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lentiviral transfer plasmid pljm1 egfp  (Addgene inc)
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Addgene inc lentiviral transfer plasmid pljm1 egfp
CAR cassettes and restriction cloning of the HuH85-encoding fragment. a : A simplified schematic of the H85CAR construct which is modified for the insertion of the HuH85-encoding DNA fragment only to develop the HuH8CAR construct. b : PCR-amplification of the HuH85-encoding fragment electrophoresed on 1% agarose gel. Lane 1: PCR amplicon (380 bp); Lane M: 100 bp DNA marker. c : Enzymatic digestion of the <t>HuH85CAR-pLJM1</t> vector electrophoresed on 1% agarose gel for the verification of the HuH8CAR cassette construction using KpnI. Lane 1: KpnI-digested HuH85CAR-pLJM1 vector; Lane M: 1000 bp DNA maker; Lane 2: undigested HuH85CAR-pLJM1 vector
Lentiviral Transfer Plasmid Pljm1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviral transfer plasmid pljm1 egfp/product/Addgene inc
Average 96 stars, based on 1 article reviews
lentiviral transfer plasmid pljm1 egfp - by Bioz Stars, 2026-03
96/100 stars
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cmv egfp lentiviral transfer plasmid  (Addgene inc)
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Addgene inc cmv egfp lentiviral transfer plasmid
CAR cassettes and restriction cloning of the HuH85-encoding fragment. a : A simplified schematic of the H85CAR construct which is modified for the insertion of the HuH85-encoding DNA fragment only to develop the HuH8CAR construct. b : PCR-amplification of the HuH85-encoding fragment electrophoresed on 1% agarose gel. Lane 1: PCR amplicon (380 bp); Lane M: 100 bp DNA marker. c : Enzymatic digestion of the <t>HuH85CAR-pLJM1</t> vector electrophoresed on 1% agarose gel for the verification of the HuH8CAR cassette construction using KpnI. Lane 1: KpnI-digested HuH85CAR-pLJM1 vector; Lane M: 1000 bp DNA maker; Lane 2: undigested HuH85CAR-pLJM1 vector
Cmv Egfp Lentiviral Transfer Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cmv egfp lentiviral transfer plasmid/product/Addgene inc
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cmv egfp lentiviral transfer plasmid - by Bioz Stars, 2026-03
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CAR cassettes and restriction cloning of the HuH85-encoding fragment. a : A simplified schematic of the H85CAR construct which is modified for the insertion of the HuH85-encoding DNA fragment only to develop the HuH8CAR construct. b : PCR-amplification of the HuH85-encoding fragment electrophoresed on 1% agarose gel. Lane 1: PCR amplicon (380 bp); Lane M: 100 bp DNA marker. c : Enzymatic digestion of the HuH85CAR-pLJM1 vector electrophoresed on 1% agarose gel for the verification of the HuH8CAR cassette construction using KpnI. Lane 1: KpnI-digested HuH85CAR-pLJM1 vector; Lane M: 1000 bp DNA maker; Lane 2: undigested HuH85CAR-pLJM1 vector

Journal: Journal of Translational Medicine

Article Title: Humanization of the antigen-recognition domain does not impinge on the antigen-binding, cytokine secretion, and antitumor reactivity of humanized nanobody-based CD19-redirected CAR-T cells

doi: 10.1186/s12967-024-05461-8

Figure Lengend Snippet: CAR cassettes and restriction cloning of the HuH85-encoding fragment. a : A simplified schematic of the H85CAR construct which is modified for the insertion of the HuH85-encoding DNA fragment only to develop the HuH8CAR construct. b : PCR-amplification of the HuH85-encoding fragment electrophoresed on 1% agarose gel. Lane 1: PCR amplicon (380 bp); Lane M: 100 bp DNA marker. c : Enzymatic digestion of the HuH85CAR-pLJM1 vector electrophoresed on 1% agarose gel for the verification of the HuH8CAR cassette construction using KpnI. Lane 1: KpnI-digested HuH85CAR-pLJM1 vector; Lane M: 1000 bp DNA maker; Lane 2: undigested HuH85CAR-pLJM1 vector

Article Snippet: Briefly, two packaging plasmids (namely, pMDLg/pRRE and pRSV/Rev), one envelope plasmid (namely, pMD2G), and the pLJM1 transfer plasmid (PLJM1-EGFP; catalog number #19319; Addgene, MA, United States) were utilized for the encapsulation of the third-generation lentiviruses in this study.

Techniques: Cloning, Construct, Modification, Amplification, Agarose Gel Electrophoresis, Marker, Plasmid Preparation